Moss Website - Experimental Methods

Homologous Recombination
(Schaefer, D. (2002)Annu. Rev. Plant Biol. 53:477-501)
1. Gene Disruption (single cross-over)
2. Gene Replacement (double cross-over)
Stable and transient RNAi.
(Bezanilla, M., Pan, A. and Quatrano, R.S. (2003). Pl. Physiol. 133:470-74)
Genomic Resources
1. ~80,000 ESTs (~21,000 contigs) database
  (http://moss.nibb.ac.jp)
2. Expression profiling ~21,000 - 60-mer Agilent chip
  (http://www.mogene.com)
3. c-DNA, g-DNA and BAC libraries
4. Genome sequencing project (2004/05) www.jgi.doe.gov/CSP/index.html