Joseph (Kuo-Hsiang) Tang PhD
Research scientist
EDUCATIONAL Background
2002 Ph.D., Biochemistry (minor: Pharmacy), University of Wisconsin, Madison, WI,
Thesis: Free radicals and lysine 5,6-aminomutase. Advisor: Dr. Perry A. Frey
1992 M.S., Chemistry (Organic Chemistry), National Taiwan University, Taipei, Taiwan.
Thesis: Free radical cyclization reactions involving acylsilanes; study of the tandem bicyclization. Advisor: Dr. Yeun-Min Tsai
1990 B.S., Chemistry, Tamkang University, Taipei, Taiwan.
ADDITIONAL TRAINING
* Genomics Training Workshop in the Institute for Genome Sciences (IGS), University of Maryland, School of Medicine (July, 2009)
* SEDFIT/SEDPHAT Analytical Ultracentrifugation Workshop, NIH, Bethesda, MD (September, 2006)
* The Higher European Research Course for Users of Large Experimental Systems (HERCULES), Neutron and Synchrotron Radiation for Biomolecular Structures and Dynamics. Grenoble, France (February¨CMarch, 2006)
Research Interests
One of my research interests is focusing at a detailed understanding of structure-function relationships of the catalytic mechanism of bio-medically and/or bio-energetically relevant enzymes. To reach these goals, I have been using a wide variety of genetic, molecular biological, biochemical, and biophysical techniques.
Moreover, I also actively explore the (energy) metabolism pathways in various photosynthetic bacteria using genomics, transcriptomics, metabolomics and biochemical approaches.
SERVICE TO PROFESSION
Proposal Review Panel Member: Oak Ridge National Laboratory Neutron Science (ORNL): 2009-present
PEER-REVIEWED PUBLICATIONS
1. Tang, K.H., Feng, X., Tang, Y. and Blankenship, R.E. Carbohydrate metabolism and carbon fixation in Roseobacter denitrificans OCh114. PLoS One accepted
2. Tang, K.H., Mansoorabadi, S., Reed, G.H. and Frey, P.A. (2009) Suicide inhibition and radical triplet intermediates in reactions of 4-thia-D- and 4-thia-L-lysine with lysine 5,6-aminomutase. Biochemistry 48, 8151-8160
3. Tang, K.H., Wen, J., Li, X. and Blankenship, R.E. (2009) Role of the AcsF protein in Chloroflexus aurantiacus. J. Bacteriol. 191, 3580-3587
4. Maity, A., Hsieh, C.P., Huang, M.H., Chen, Y.H., Tang, K.H., Behshad, E., Frey, P.A. and Ke, S.C. (2009) Evidence for conformational movement and radical mechanism in the reaction of 4-thia-L-lysine with lysine 5,6-aminomutase. J. Phys. Chem. B, in press
5. Tang, K.H., Niebuhr, M., Tung, C.S., Chen, S.C., Chou, C.C., and Tsai, M.D. Mismatched dNTP incorporation by DNA polymerase does not proceed via globally different conformational pathways. Nucleic Acids Res. 36, 2948-2957 (2008) (Co-corresponding author).
6. Tang, K.H. and Tsai, M. D. Structure and Function of 2:1 DNA polymerase·DNA complexes. J. Cell Physiol. 216, 315-320 (2008).
7. Tang, K.H., Niebuhr, M., Aulabaugh, A., and Tsai, M.D. Solution structures of 2:1 and 1:1 DNA polymerase-DNA complexes probed by ultracentrifugation and small-angle x-ray scattering. Nucleic Acids Res. 36, 849-860 (2008).
8. Tang, K.H. and Frey, P.A. Suicide Inhibition and B12-Radical Triplets in the Reactions of 4-Thia-D- and 4-Thia-L-lysine with Lysine 5,6-Aminomutase.Biochem. J. under review.
9. Tang, K.H., Guo, H., Yi, W., Tsai, M.D., and Wong, G.P. Investigation of conformational states of Wzz and the Wzz•O-antigen complex under near-physiological conditions. Biochemistry 46, 11744-11752 (2007).
10. Showalter, A.K., Lamarche, B.J., Bakhtina, M., Su, M.I., Tang, K.H., and Tsai, M.D. Mechanistic comparison of high-fidelity and error-prone DNA polymerases and ligases involved in DNA repair. Chem. Rev. 106, 340-360 (2006).
11. Tang, K.H., Liao, F.Y. and Tsai, Y.M. The study of intramolecular tandem radical cyclizations of acylsilanes with radicalphiles attached on silicon. Tetrahedron 61, 2037-2045 (2005).
12. Berkovitch, F, Behshad, E., Tang, K.H., Enns, E.A., Frey, P.A. and Drennan, C.L. A locking mechanism preventing radical damage in the absence of substrate, as revealed by the x-ray structure of lysine 5,6-aminomutase. Proc Natl Acad Sci U S A 101, 15870-15875 (2004).
13. Karbstein, K., Tang, K.H. and Herschlag, D. A base triple in the Tetrahymena group I core affects the reaction equilibrium via a threshold effect. RNA 10, 1730-1739 (2004).
14. Tang, K.H., Casarez, A.D., Wu, W. and Frey, P.A. Kinetic and biochemical analysis of the mechanism of action of lysine 5,6-aminomutase. Arch Biochem Biophys 418, 49-54 (2003).
15. Tang, K.H., Harms, A. and Frey, P.A. Identification of a novel pyridoxal 5´-phosphate binding site in adenosylcobalamin-dependent lysine 5,6-aminomutase from Porphyromonas gingivalis. Biochemistry 41, 8767-8776 (2002).
16. Tang, K.H., Chang, C.H. and Frey, P.A. Electron transfer in the substrate-dependent suicide inactivation of lysine 5,6-aminomutase. Biochemistry 40, 5190-5199 (2001).
17. Jiaang, W.T., Lin, H.C., Tang, K.H., Chang, L.B. and Tsai, Y.M. The study of kinetics of intramolecular radical cyclizations of acylsilanes via the intramolecular competition method. J. Org. Chem. 64, 618-628 (1999).
18. Chang, S.Y., Jiaang, W.T., Cherng, C.D., Tang, K.H., Huang, C.H. and Tsai, Y.M. The scope and limitations of intramolecular radical cyclizations of acylsilanes with alkyl, aryl, and vinyl radicals. J. Org. Chem. 62, 9089-9098 (1997).
19. Tsai, Y.M., Tang, K.H. and Jiaang, W.T. Group transfer from silicon to carbon via tandem radical cyclizations of acylsilanes. Tetrahedron Letters 37, 7767-7770 (1996).
20. Tsai, Y.M., Tang, K.H. and Jiaang, W.T. Radical cyclizations of bromo acylsilanes and intramolecular trapping of the rearranged alpha-silyloxy radicals. Tetrahedron Letters 34, 1303-1306 (1993).
INVITED TALK AND ORAL PRESENTATIONS
1. The 13th International Symposium on Phototrophic Prokaryotes, Montr¨¦al, QC, Canada (August, 2009)
2. The 40th ACS Central Regional Meeting, Columbus, OH (June, 2008)
3. 11th Symposium on Recent Advances in Biophysics, Taipei, Taiwan (May, 2006)
